Melatonin Attenuates Ischemia/Reperfusion Injury of Rat Flap through the Rip3-Mlkl-Mptp Necroptotic Pathway

Wang Y, Zhou M, Wu Y and Rui Y*

Department of Orthopedics Surgery, Wuxi No. 9 People’s Hospital, Soochow University, China

^*Correspondance to: Yongjun Rui 

 PDF  Full Text DOI: 10.25107/2474-1647.3443

Abstract

In this study, we investigated the protective effects of melatonin on Ischemia/Reperfusion (I/R) injury model in rat flaps and its possible mechanism. In total, 60 male Sprague-Dawley rats were randomly divided into three groups: Sham operation group (SH group), normal saline group and melatonin group. After I/R treatment, rats in the operation group were treated with normal saline and melatonin. The flap survival rate was calculated on the 7th day after operation. Immunohistochemistry and RT-qPCR were used to detect the expression of RIP3, MLKL and MPTP. Compared with the normal saline group, the flap survival area of in the SH group and the melatonin group was larger. The expression of RIP3, MLKL and MPTP were highly expressed in the normal saline group, which was significantly decreased in the melatonin group. In addition, the expression of necrosis factor in the SH group and the melatonin group were similar and significantly lower than that in the normal saline group, suggesting that the protective effect of melatonin on skin I/R necrosis in rats may be associated with the necrotic pathway.

Keywords

Cite the article

Wang Y, Zhou M, Wu Y, Rui Y. Melatonin Attenuates Ischemia/ Reperfusion Injury of Rat Flap through the Rip3-Mlkl-Mptp Necroptotic Pathway. Clin Surg. 2022; 7: 3443.