Journal Basic Info

  • Impact Factor: 1.995**
  • H-Index: 8
  • ISSN: 2474-1647
  • DOI: 10.25107/2474-1647
**Impact Factor calculated based on Google Scholar Citations. Please contact us for any more details.

Major Scope

  •  Oral and Maxillofacial Surgery
  •  Neurological Surgery
  •  Urology
  •  Gynecological Surgery
  •  Ophthalmic Surgery
  •  Endocrine Surgery
  •  Emergency Surgery
  •  Pediatric Surgery

Abstract

Citation: Clin Surg. 2020;5(1):2713.Research Article | Open Access

Curcumin-Induced Adipose-Derived Stem Cell Exosomes Alter Adipogenic Differentiation Capabilities

Jingping Wang1, Ziwan Ji1, Yao Qian1, Hao Chen1, Yucang He1, Xuling Lv1, Zikai Zhang1, Tian Li1, Liqun Li1 and Ming Lin2*

1First Affiliated Hospital of Wenzhou Medical University, China
2Department of Plastic Surgery, Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, China

*Correspondance to: Ming Lin 

 PDF  Full Text DOI: 10.25107/2474-1647.2713

Abstract

Adipose-Derived Stem Cells (ADSCs) have multi-directional differentiation potential; however, their efficiency in differentiating into mature adipocytes remains low, which is a limitation for clinical and research applications of stem cell therapy. Curcumin has been reported to promote the proliferation of adipose-derived stem cells, but the effects of curcumin on the secretion of ADSC exosomes and the function of exosomes on adipogenic differentiation have not been assessed. Therefore, the objective of the present study was to investigate the exosome of ADSCs stimulated with curcumin and the consequence for adipogenic differentiation. Human ADSCs were isolated by the adherence method and characterized according to adipogenic, osteogenic, and chondrogenic differentiation ability. Exosomes were extracted from the supernatant of the third-generation ADSCs, and characterized by transmission electron microscopy and western blotting. Curcumin (10 μmol/L) was added to the complete culture medium 3 days before the extraction, and the influence of the exosomes on adipogenic differentiation was assessed. The human ADSCs showed mesenchymal stem cell characteristics and could differentiate into mature adipocytes, osteoblasts, and chondrocytes. Exosomes secreted by curcumin-treated ADSCs showed typical exosome characteristics and enhanced cell migration compared with that of exosomes in the untreated group. Moreover, curcumin-treated exosomes increased the number of fat cells produced after adipogenic differentiation but not the volume of fat cells. Overall, these results indicate a beneficial role of exosomes from ADSCs on adipogenesis and ADSC activity, providing new insight into the processes of fat accumulation and potential new strategies for improving the expansion of ADSCs for their clinical use in regenerative medicine.

Keywords

Exosome; Adipose-derived stem cell; Fat accumulation; Differentiation; Tissue regeneration; Curcumin

Cite the article

Wang J, Ji Z, Qian Y, Chen H, He Y, Lv X, et al. Curcumin-Induced Adipose- Derived Stem Cell Exosomes Alter Adipogenic Differentiation Capabilities. Clin Surg. 2020; 5: 2713..

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