The Effect of Vascular Endothelial Growth Factor in the Improvement of Microcirculation Disturbance in Rats with Severe Acute Pancreatitis

Palikhe M, Zhan J, Jha RK and Zhang X

Department of Gastroenterology, The Second Affiliated Hospital of Xi’an Jiaotong University, China
Department of Gastroenterology, Xi’an Medical University, China

^*Correspondance to: Zhang Jun 

 PDF  Full Text DOI: 10.25107/2474-1647.2175

Abstract

Objective: To observe the function of Vascular Endothelial Growth Factor (VEGF) in the improvement of microcirculation disturbance in rats with Severe Acute Pancreatitis (SAP).Methods: 120 adult male Sprague-Dawley (SD) rats were randomly divided into four groups, the Sham Operation (SO) SAP VEGF treatment or VEGF antibody (anti-VEGF) treatment group. The mortality rates were counted at 24 hr; the dry/wet ratios of tissues were measured in the lung, small intestine, and liver; changes in the ultrastructure of the microcirculation in pancreatic and lung tissue were observed using a Transmission Electron Microscope (TEM), and the levels of VEGF, Nitric Oxide (NO), Endothelin (ET), and trypsin were measured using an Enzyme-Linked Immunosorbent Assay (ELISA).Results: The mortality in SO group at 24 hr was 0%, SAP group was 90% (9/10), anti-VEGF group was 80% (8/10), and VEGF group was only 10% (1/10). The dry/wet ratios of all tissues in the VEGF group were significantly lower than those in the SAP and anti-VEGF groups (P<0.05). Results from the TEM showed that the SAP and anti-VEGF groups exhibited obvious pathological changes. The serum ET, NO, and trypsin levels in the SAP and anti-VEGF groups were significantly higher than those in the SO group (P<0.05).Conclusion: VEGF could improve microcirculation disturbance in SAP rats through the repair of the vascular endothelial barrier and maintenance of vascular endothelial function to further reduce the mortality of rats, which might become a new breakthrough in SAP treatment in the future.

Keywords

Severe acute pancreatitis; Vascular endothelial growth factor; Microcirculation disturbance; Multiple organ damage

Cite the article

Palikhe M, Zhan J, Jha RK, Zhang X. The Effect of Vascular Endothelial Growth Factor in the Improvement of Microcirculation Disturbance in Rats with Severe Acute Pancreatitis. Clin Surg. 2018; 3: 2175.