Tracking Down the Cause of Necrotizing Fasciitis in a Patient with Negative Cultures

Bas P Vierhout, Evert van Zanten, Guido Wisselink, Mirjam AMD Kooistra-Smid and Alewijn Ott

Department of Surgery, Wilhelmina Ziekenhuis, Assen, Netherlands
Department of Medical Microbiology, Certe, Groningen, Netherlands
Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands

^*Correspondance to: Alewijn Ott 

 PDF  Full Text DOI: 10.25107/2474-1647.2011

Abstract

In a patient suspected of lower abdominal necrotizing fasciitis cultures remained negative, possibly because broad spectrum antibiotics had already been given before tissue for culture was obtained. 16S-23S rDNANext Generation Sequencing showed that 99.7% of bacterial DNA was of Streptococcus pyogenes. 16S-23S rDNA can replace culture for identification of bacteria, also in polymicrobial infection.

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Cite the article

Vierhout BP, van Zanten E, Wisselink G, Kooistra-Smid MAMD, Ott A. Tracking Down the Cause of Necrotizing Fasciitis in a Patient with Negative Cultures. Clin Surg. 2018; 3: 2011.