Cholecystokinin-8 Treatment of Pigs with Induced Acute Pancreatitis Significantly Reduces Acinar Necrosis and Edema of Pancreatic Tissue

Katharina Grupp, Sarah Bonk, Annika Poppe, Lena Seifert, Karin Wodack, Constantin Trepte, Matthias Reeh, Andreas Gocht, Oliver Mann, Jakob R Izbicki and Kai Bachmann

Department of General, Visceral and Thoracic Surgery, University Medical Centre Hamburg-Eppendorf, Germany
Centre of Anesthesiology and Intensive Care Medicine, University Medical Centre Hamburg-Eppendorf, Germany
Joint Practice of Pathology, Germany

^*Correspondance to: Katharina Grupp 

 PDF  Full Text DOI: 10.25107/2474-1647.1988

Abstract

Objective: Acute pancreatitis is an inflammatory process of the pancreas and a leading cause of hospitalization amongst gastrointestinal disorders. Previously, cholecystokinin (CCK) has been described to play a role in regeneration of pancreas. This study was undertaken to get more insights in the function of cholecystokinin octapeptide (CCK - 8) during induced pancreatitis in an animal model.Methods: In this study, acute pancreatitis was induced in 38 pigs. Two hours after the induction of acute pancreatitis, the animals were grouped according to the melatonin treatment into the following two groups: group 1/CCK - 8 group and group 2/non - CCK - 8 groups. Intraoperative clinical data, postoperative blood parameters and ‘Porcine Well-Being’ (PWB) score, as well as post - mortal histopathological data were analysed.Results: At baseline, physiologically parameters of the pigs of both groups were comparable. No differences were observed regarding the overall survival of animals (p=0.97). Postoperative PWB score were significantly better in animals treated with CCK - 8 as compared to the control group (p=0.029). Moreover, histopathological analysis of the pancreatic tissue revealed that acinar necrosis and edema were significant reduced in the CCK - 8 group in comparison to the control group (p=0.016 and p=0.019).
Conclusion: CCK - 8 treatments reduces acinar necrosis and edema of pancreatic tissue after induction of an acute pancreatitis in pigs. Thus, it can be speculated that CCK - 8 may be useful as a therapeutic medical treatment of severe acute pancreatitis.

Keywords

Acute pancreatitis; Experimental model; Cholecystokinin - 8; CCK-8

Cite the article

Grupp K, Bonk S, Poppe A, Seifert L, Wodack K, Trepte C, et al. Cholecystokinin-8 Treatment of Pigs with Induced Acute Pancreatitis Significantly Reduces Acinar Necrosis and Edema of Pancreatic Tissue. Clin Surg. 2018; 3: 1988.