Quantification of Serum Levels of Two Potential Biomarkers and Clinical Features in Osteoarthritis Patients

Takashi Kuroiwa, Hiroshi Ageta, Daiki Ikeda, Mitsuhiro Morita, Kunihiro Tsuchida and Harumoto Yamadaz

Department of Orthopaedic Surgery, Fujita Health University Hospital, Toyoake, Aichi, Japan
Division for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan

^*Correspondance to: Harumoto Yamadaz 

 PDF  Full Text DOI: 10.25107/2474-1647.1378

Abstract

Introduction: Our previous proteomics analysis of human articular cartilage demonstrated that levels of leukocyte cell-derived chemotaxin 2 (LECT2) and peroxiredoxin 6 (PRDX6) in the cartilage differed significantly between osteoarthritis (OA) and control patients. LECT2 and PRDX6 levels were significantly increased and decreased, respectively, in articular cartilages from OA patients. In the present study, we evaluated the clinical significance of these findings by measuring serum levels of LECT2 and PRDX6 in OA patients.Materials and
Methods: OA samples were obtained from 73 patients with severe hip and knee OA. Control samples were obtained from 16 patients with femoral neck fracture (Fr). Serum LECT2 and PRDX6 levels were measured by enzyme-linked immunosorbent assay.Results: Serum LECT2 and PRDX6 levels between OA and Fr patients showed the same trends with our previous study. Intriguingly, serum PRDX6 level was markedly elevated in OA patients 3 months after surgery as compared to before surgery. In addition, serum LECT2 and blood urea nitrogen (BUN) levels were correlated, while serum PRDX6 level was correlated with BUN, sodium, and potassium levels.Conclusion: Our results indicate that PRDX6 will not only be a candidate biomarker for diagnosis of OA before operation, but also a biomarker for the evaluation of healing process of OA.

Keywords

Osteoarthritis; LECT2; PRDX6

Cite the article

Kuroiwa T, Ageta H, Ikeda D, Morita M, Tsuchida K, Yamadaz H. Quantification of Serum Levels of Two Potential Biomarkers and Clinical Features in Osteoarthritis Patients. Clin Surg. 2017; 2: 1378.