Study of the Biological Effect of Bufalin on Anti-Proliferation, Adhesion and Invasion in Liver Cancer Cells

Sheng X, Zhu PF and Qin JM

Department of Pathology, Second Military Medical University, China
Department of General Surgery, Second Military Medical University, China

^*Correspondance to: JIanmin Qin 

 PDF  Full Text DOI: 10.25107/2474-1647.1363

Abstract

Background: Recurrence and metastasis are the main factors which seriously influence the therapeutic effect and long-term survival for hepatocellular carcinoma (HCC). Bufalin is a kind of topology isomerase II inhibitors. The pharmacological effects of bufalin are anti-tumor, anticoagulant, analgesia, enhanced systole and immunity. Therefore, in the present study, we explore the migration, invasion and adhesion influence of bufalin on the liver cancer cells, and provide better theoretical basis for bufalin on preventing and treating the recurrence and metastasis of HCC.Methods: The human high metastasis potential LM3 hepatoma cells (HCC-LM3) were cultured in vitro. The influence of bufalin on HCC-LM3 cell proliferation was detected by the CCK-8 living cells staining technique, and the migration, invasion and adhesion influence of bufalin on the HCC-LM3 cells were detected by the Trans well Chambers technique.Results and
Discussion: The results showed that bufalin can inhibit the growth of liver cancer cells with time and concentration of dual dependence. With the increase of dose concentration, the inhibitive effect of bufalin on liver cancer cell adhesion, migration and invasion ability significantly enhanced. Bufalin is a kind of agent being potential application value to inhibit the recurrence and metastasis of HCC.

Keywords

Hepatocellular carcinoma; Bufalin; Proliferation; Metastasis

Cite the article

Sheng X, Zhu PF, Qin JM. Study of the Biological Effect of Bufalin on Anti-Proliferation, Adhesion and Invasion in Liver Cancer Cells. Clin Surg. 2017; 2: 1363.