The Association between Microsatellite Alteration and Survival of Oral Cavity Squamous Cell Carcinoma Patients from an Endemic Betel Quid Chewing Area

Shih-An Liu, Chen-Chi Wang, Rong-San Jiang, Wen-Yi Wang and Jin-Ching Lin

Department of Otolaryngology, Taichung Veterans General Hospital, Taiwan
Department of Radiation Oncology, Taichung Veterans General Hospital, Taichung, Taiwan
Faculty of Medicine, School of Medicine, National Yang-Ming University, Taiwan
Department of Nursing, Hung Kuang University, Taiwan
Department of Medical Research, China Medical University, Taiwan

^*Correspondance to: Jin-Ching Lin 

 PDF  Full Text DOI: 10.25107/2474-1647.1311

Abstract

Background: The incidence of microsatellite alterations at the endemic betel quid chewing area and its association with the survival of patients with oral cavity squamous cell carcinoma (OCSCC) is not clear. Here we studied their possible relationship.
Methods: Subjects were 135 patients with histological confirmed OCSCC. From these patients we obtained their cancerous tissues, corresponding surgical margins, and peripheral blood samples. From these specimens, we analyzed the microsatellite alterations base on 10 oligonucleotide markers. Specifically, specimens were assessed by automatic fragment analysis following amplification by polymerase chain reactions.Results: Of these specimens, 45 (33.3%) showed microsatellite instability (MSI) and 78 (57.8%) showed loss of heterozygosity (LOH) for at least one marker. Using Kaplan-Meier’s analysis method, microsatellite alterations of patients did not associate with their disease-specific survival. However, the presence of MSI in surgical margins of the cancer increased the risk of local recurrence (odds ratio: 7.49; 95% confidence interval: 3.34 ~ 16.80; P < 0.001).Conclusion: The prognosis of OCSCC patients was not associated with microsatellite alterations in region where betel quid chewing is prevalent. However, genomic examination of surgical margin can possibly find out OCSCC patients who are prone to develop local recurrence.

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Cite the article

Liu S-A, Wang C-C, Jiang R-S, Wang W-Y, Lin J-C. The Association between Microsatellite Alteration and Survival of Oral Cavity Squamous Cell Carcinoma Patients from an Endemic Betel Quid Chewing Area. Clin Surg. 2017; 2: 1311.