Linghe Zang1, Wusheng Liu2, Yuwei Zhang2, Mengzhen Zhang1, Jing Zhao1, Xuemiao Yang1, Yongxue Li1, Jiayu Zhang1, Yibo Shang1, Pengfei Wang1, Zhenbo Yang1, Liping Zhou1, Yanling Chi1, Zhaoqing Meng2* and Jincai Lu1*
1Institute of Life science and Bio-Pharmaceuticals, Pharmaceutical College, Shenyang Pharmaceutical University, China
2Institute of Traditional Chinese Medicine, Shandong Hongjitang Pharmaceutical Group Co., Ltd., China
Objective: Qianliexin capsule (QLX), a Traditional Chinese Medicine, has been widely used to treat Chronic Nonbacterial Prostatitis (CNP) in China. Here, metabolic profiling was utilized to clarify the bio-targets of QLX in CNP treatment. Methods: An estradiol-induced prostatitis rat model was used to mimic the hormonal imbalanceinduced CNP. The urine and serum were collected to analyze metabolic profiles by Ultra-High- Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). Results: An anti-inflammatory role for QLX was seen in the estradiol-induced prostatitis rats, and the Lower Urinary Tract Symptoms (LUTS) were relieved by QLX. The unique metabolic profiling in CNP was significantly changed by addition of QLX. Thirty-nine metabolites identified in serum and urine, that are involved in multiply pathways, were significantly reversed by QLX in the CNP rats. Among them, glycerophospholipid metabolism, sphingolipid metabolism and fructose and mannose metabolic pathways were the most significantly influenced by QLX and were closely associated with the anti-inflammatory role of QLX in CNP. Conclusion: The changed metabolic profile after QLX addition may be associated with the pharmacologic function of QLX in CNP.
Qianliexin capsule; Chronic abacterial prostatitis; Metabolomics; Biomarker
Zang L, Liu W, Zhang Y, Zhang M, Zhao J, Yang X, et al. Exploration of the Mechanism of Qianliexin Capsule against Chronic Nonbacterial Prostatitis Using Metabolomics. Clin Surg. 2021; 6: 3316.