Long Noncoding RNA LINC00520 Accelerates the Progression of Colorectal Cancer by Serving as a Competing Endogenous RNA of Microrna-577 to Increase HSP27 Expression

Xi-Han Jin1#, Yong-Gang Hong2#, Peng Li1, Li-Qiang Hao2* and Ming Chen3*

Department of Colorectal Surgery, Jinhua Municipal Central Hospital, Jinhua Hospital of Zhejiang University, China
2Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, China
3Department of General Surgery, Taizhou State Hospital of Zhejiang Province, Medical College of Taizhou University, China
#Both authors contributed equally to this work

^*Correspondance to: Li-Qiang Hao 

 PDF  Full Text DOI: 10.25107/2474-1647.2745

Abstract

The Long Noncoding RNA (lncRNA) LINC00520 is an important modulator of the oncogenicity of multiple human cancers. However, whether LINC00520 is involved in the malignant characteristics of Colorectal Cancer (CRC) has not been extensively studied until recently. Therefore, the present study aimed to detect LINC00520 expression in CRC and evaluate its clinical significance in patients with CRC. Functional experiments were conducted to test the biological roles and underlying mechanisms of LINC00520 in CRC progression. In this study, high LINC00520 expression was verified in CRC tissues and cell lines, and this high expression was associated with patients’ unfavorable clinico-pathological parameters and shorter overall survival and disease-free survival. Functionally, interference of LINC00520 resulted in a significant decrease of CRC cell proliferation, migration, colony formation ability, and invasion. Mechanistically, LINC00520 functioned as a competing endogenous RNA by sponging microRNA-577 (miR-577) and thereby increasing Heat shock protein 27 (HSP27) expressions. Rescue experiments revealed that inhibiting miR-577 or restoring HSP27 could abrogate the effects of LINC00520 silencing on malignant phenotypes of CRC. LINC00520 functioned as an oncogenic lncRNA in CRC, and it facilitated CRC progression by regulating the miR-577/HSP27 axis, suggesting that the LINC00520/miR-577/HSP27 axis is an effective target in anticancer management.

Keywords

LINC00520; Colorectal Cancer; Microrna-577; Heat Shock Protein 27

Cite the article

Jin X-H, Hong Y-G, Li P, Hao L-Q, Chen M. Long Noncoding RNA LINC00520 Accelerates the Progression of Colorectal Cancer by Serving as a Competing Endogenous RNA of Microrna-577 to Increase HSP27 Expression. Clin Surg. 2020; 5: 2745..