Li XJ1, Yao YF1, Ren Z2, Bao J3* and Yu Q1
1Department of General Surgery, Jiangsu Cancer Hospital, China
2Department of Pathology, Jiangsu Cancer Hospital, China
3Department of Medical Oncology, Jiangsu Cancer Hospital, China
Background/Aims: This study aims to investigate circRNA expression profiles and their potential biological functions in Triple Negative Breast Cancer (TNBC).
Methods: High-throughput RNA sequencing was used to assess circRNA expression profiles in TNBC, and Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) was used to validate dysregulated circRNA s. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were employed to determine the biological functions of differentially expressed circRNA s. Bioinformatic analyses were applied to predict interactions between circRNA s and microRNAs (miRNAs), and a circRNA -miRNA-mRNA network was constructed using Cytoscape software.
Results: We identified a number of differentially expressed circRNA s in TNBC tissues compared with paired normal tissues, with chr11:32589546:32596047:+, chr18:36195256:36203189:+, chr7:22266963:22318037:-, chr9:22046750:22064018:+, and chr10:836402:845055:- being up regulated, and chr12: 55700898:55701154:-, chr15:54012647:54015886:+, chr5:55467868:55491114:- , chr4:61934839:62044549:+ and chr7:152145152:152149152:- being down regulated. These findings were confirmed by qRT-PCR. GO and KEGG pathway analyses showed that some of these circRNA s are related to cancers. Additionally, bioinformatic analyses revealed a potential competingendogenous- RNA-regulating network among circRNA s, miRNAs, and mRNAs.
Conclusion: Our study results depict the landscape of circRNA expression profiles in TNBC and also provide potential biomarkers for TNBC. Further functional and mechanistic studies of this circRNA s may improve our understanding of TNBC tumorigenesis.
Li XJ, Yao YF, Ren Z, Bao J, Yu Q. Systematical Analysis of Circular RNAs in Triple Negative Breast Cancer. Clin Surg. 2019; 4: 2450.